RYBP Monoklonaler Antikörper

RYBP Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C6G7

Kat-Nr. : CL488-68130

Synonyme

RYBP, YEAF1, YY1 and E4TF1-associated factor 1, 2C6G7, AAP1, APAP 1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DED associated factor, DEDAF, DED-associated factor, RING1 and YY1-binding protein

---

• 

  FC experiment of A431 using CL488-68130

  1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RYBP (CL488-68130, Clone:2C6G7) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68130 bindet in FC (Intra) RYBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RYBP fusion protein Ag31715
• Vollständiger Name: RING1 and YY1 binding protein
• Berechnetes Molekulargewicht: 228 aa, 25 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC014959
• Gene symbol: RYBP
• Gene ID (NCBI): 23429
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]