Rabbit IgG control Polyklonaler Antikörper

Rabbit IgG control Polyklonal Antikörper für FC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

FC, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-30000

Synonyme

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

---

• 

  FC experiment of NIH/3T3 using CL555-30000

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Rabbit IgG control (CL555-30000) (red), control used were unstained NIH/3T3 cells (Blue). Positive control is CoraLite®555 Calreticulin (CL555-27298) (Green). Cells were fixed with permeabilized with Transcription Factor Staining Buffer Kit (PF00011). and permeabilized Flow Perm Buffer (PF00011).

• 

  FC experiment of MCF-7 using CL555-30000

  1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite®555 Anti-Rabbit IgG control (CL555-30000), or 0.4 ug CoraLite®555-conjugated EPCAM Rabbit PolyAb (CL555-21050), or unstained (blue). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-30000 bindet in FC, FC (Intra) Rabbit IgG control und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Rabbit IgG control
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.