Ribosomal protein L4 Rekombinanter Antikörper

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• KD/KO Validated

Ribosomal protein L4 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3K6

Kat-Nr. : 80959-1-RR

Synonyme

RPL4, 3K6, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

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  WB analysis using 80959-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 80959-1-RR

  WB result of Ribosomal protein L4 antibody (80959-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HEK-293 cells.

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  IP experiment of HeLa using 80959-1-RR

  IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug.

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  IHC staining of human stomach cancer using 80959-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 80959-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 80959-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 80959-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80959-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Ribosomal protein L4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ribosomal protein L4 fusion protein Ag28728
• Vollständiger Name: ribosomal protein L4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC009888
• Gene symbol: Ribosomal protein L4
• Gene ID (NCBI): 6124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.