S100B Rekombinanter Antikörper

S100B Rekombinant Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1E2

Kat-Nr. : 82271-8-RR

Synonyme

S100 beta, 1E2, Protein S100 B, Protein S100-B, S100 calcium-binding protein B

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  WB analysis using 82271-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82271-8-RR (S100B antibody) at dilution of 1:5150 incubated at room temperature for 1.5 hours.

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  IHC staining of Human appendicitis using 82271-8-RR

  Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human appendicitis using 82271-8-RR

  Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 82271-8-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of rat brain using 82271-8-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IF Staining of rat brain using 82271-8-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using S100B antibody (82271-8-RR, Clone: 1E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 82271-8-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using S100B antibody (82271-8-RR, Clone: 1E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 82271-8-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using S100B antibody (82271-8-RR, Clone: 1E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 82271-8-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using S100B antibody (82271-8-RR, Clone: 1E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A375 using 82271-8-RR

  1x10^6 A375 cells were intracellularly stained with 0.25 ug S100B Recombinant antibody (82271-8-RR, Clone:1E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82271-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82271-8-RR against Human S100 Beta were performed. The affinity constant is 38.5 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, A375-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10300
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82271-8-RR bindet in WB, IHC, IF-P, FC (Intra), ELISA S100B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: S100B fusion protein Ag7440
• Vollständiger Name: S100 calcium binding protein B
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC001766
• Gene symbol: S100 Beta
• Gene ID (NCBI): 6285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.