S100A10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A10 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(70)

Kat-Nr. : 11250-1-AP

Synonyme

ANX2L, ANX2LG, CAL1L, Calpactin 1 light chain, Calpactin I light chain

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  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11250-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human colon using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human skin using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human skin using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 11250-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11250-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 11250-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HaCaT-Zellen, HeLa-Zellen, HT-29-Zellen, humanes Lungengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Zervixkarzinomgewebe, humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Prostatahyperplasie-Gewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 43 publications below
• IHC: See 8 publications below
• IF: See 36 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below

Produktinformation

11250-1-AP bindet in WB, IHC, IF/ICC, FC, IP, ELISA S100A10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A10 fusion protein Ag1779
• Vollständiger Name: S100 calcium binding protein A10
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC015973
• Gene symbol: S100A10
• Gene ID (NCBI): 6281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Publikationen

Species	Application	Title
		Matrix Biol; Antibodies and methods for immunohistochemistry of extracellular matrix proteins.; Authors - Steffen Rickelt
mouse	WB	Cell Mol Immunol; Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.; Authors - Yunwei Lou
mouse	WB,IF	Int J Biol Macromol; Astrocyte-conditional knockout of MOB2 inhibits the phenotypic conversion of reactive astrocytes from A1 to A2 following spinal cord injury in mice; Authors - Xin Tao
mouse	IF	Acta Pharmacol Sin; VEGFD/VEGFR3 signaling contributes to the dysfunction of the astrocyte IL-3/microglia IL-3Rα cross-talk and drives neuroinflammation in mouse ischemic stroke; Authors - Shuai Wang
rat	IF	JCI Insight; Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity; Authors - Tyler B Waltz
mouse	WB,IF	Phytomedicine; Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis; Authors - Zixian Zhou