S100A16 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A16 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11456-1-AP

Synonyme

AAG13, DT1P1A7, Protein S100 A16, Protein S100 F, S100A16, S100F

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Galerie der Validierungsdaten

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  WB analysis of A431 using 11456-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11456-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 11456-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 11456-1-AP (S100A16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, DU 145-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

11456-1-AP bindet in WB, IP, IHC, IF, ELISA S100A16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A16 fusion protein Ag2023
• Vollständiger Name: S100 calcium binding protein A16
• Berechnetes Molekulargewicht: 103 aa, 12 kDa
• Beobachtetes Molekulargewicht: 9-12 kDa
• GenBank-Zugangsnummer: BC019099
• Gene symbol: S100A16
• Gene ID (NCBI): 140576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Int J Biol Sci; S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development; Authors - Yifang Hu ; KD Validated
mouse	WB,IF	Cell Mol Life Sci; S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.; Authors - Yifei Sun ; KO Validated
human	WB,IF	FASEB J; Brain microvascular endothelial cell exosome-mediated S100A16 up-regulation confers small-cell lung cancer cell survival in brain.; Authors - Zhi-Hua Xu
human	WB,IP	Front Cell Dev Biol; Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer; Authors - Xiaoying You ; KD Validated
human	IHC, WB	Biomolecules; ADAMTS19 Suppresses Cell Migration and Invasion by Targeting S100A16 via the NF-κB Pathway in Human Gastric Cancer.; Authors - Yingming Jiang
human	WB,IHC,IP	PLoS One; S100A14 Interacts with S100A16 and Regulates Its Expression in Human Cancer Cells.; Authors - Sapkota Dipak D