S100A2 Polyklonaler Antikörper

S100A2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15454-1-AP

Synonyme

CAN19, Protein S 100L, Protein S100 A2, Protein S100-A2, Protein S-100L

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  WB analysis using 15454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15454-1-AP (S100A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15454-1-AP (S100A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human bowen disease using 15454-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15454-1-AP (S100A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 15454-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15454-1-AP (S100A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15454-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using S100A2 antibody (15454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 15454-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.4 ug S100A2 Polyclonal antibody (15454-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HaCaT-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: human bowen disease; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15454-1-AP bindet in WB, IHC, IF/ICC, ELISA S100A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A2 fusion protein Ag7719
• Vollständiger Name: S100 calcium binding protein A2
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC002829
• Gene symbol: S100A2
• Gene ID (NCBI): 6273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A2 (S100 calcium-binding protein A2) is also named as S100L and CAN19. S100A2, a member of the S100 protein family, is more highly expressed in endometrial carcinoma tissues than in normal tissues and was correlated with worse survival (PMID: 35042454). S100A2 has a tumor suppressor function. S100A2 is highly upregulated in the epidermis under inflammatory conditions and in drug eruptions, in addition to inflammatory skin diseases such as atopic dermatitis and psoriasis (PMID: 35885660).