S100A6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A6 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 10245-1-AP

Synonyme

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10245-1-AP

  WB result of S100A6 antibody (10245-1-AP, 1:30000) with si-Control and si-S100A6 transfected HeLa cells..

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  WB analysis using 10245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10245-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10245-1-AP

  IP result of anti-S100A6(IP:10245-1-AP, 4ug; Detection:10245-1-AP 1:5000) with A549 cells lysate 840 ug.

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  IHC staining of human stomach cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10245-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10245-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A6 antibody (10245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10245-1-AP

  Immunofluorescent analysis of MCF-7 cells, using S100A6 antibody 10245-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of MCF-7 using 10245-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human S100A6 (10245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

10245-1-AP bindet in WB, IP, IHC, IF, FC, ELISA S100A6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A6 fusion protein Ag0385
• Vollständiger Name: S100 calcium binding protein A6
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC001431
• Gene symbol: S100A6
• Gene ID (NCBI): 6277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.

Publikationen

Species	Application	Title
human	IF	Cell; Cellular architecture of human brain metastases.; Authors - Hugo Gonzalez
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	IHC,IF	Sci Transl Med; Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.; Authors - Bradley N Smith
human	IF	Cancer Commun (Lond); Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.; Authors - Chaoji Shi
human	WB,IHC	Mol Carcinog; S100A6 promotes cell proliferation in human nasopharyngeal carcinoma via the p38/MAPK signalling pathway.; Authors - Anchuan Li ; KD Validated
human	WB,IHC	Neurobiol Aging; Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.; Authors - Elisa Teyssou