S100A6 Monoklonaler Antikörper

S100A6 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3D11C8

Kat-Nr. : 66098-1-Ig

Synonyme

3D11C8, CABP, CACY, Calcyclin, Growth factor-inducible protein 2A9

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  WB analysis of HeLa using 66098-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66098-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 66098-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A6 antibody (66098-1-Ig, Clone: 3D11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66098-1-Ig bindet in WB, IHC, IF/ICC, ELISA S100A6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100A6 fusion protein Ag17606
• Vollständiger Name: S100 calcium binding protein A6
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC001431
• Gene symbol: S100A6
• Gene ID (NCBI): 6277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. Although S100A6 is a cytoplasmic protein, it can be located in the nuclear and cytoplasmic membranes in the presence of Ca2+ (PMID: 37670392).

Publikationen

Species	Application	Title
human	WB	Open Med (Wars); S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.; Authors - Dongqiang Song