S100A8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A8 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(44)

Kat-Nr. : 15792-1-AP

Synonyme

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

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  WB analysis of THP-1 using 15792-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15792-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 15792-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15792-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15792-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15792-1-AP

  IP result of anti-S100A8 (IP:15792-1-AP, 4ug; Detection:15792-1-AP 1:600) with MCF-7 cells lysate 1400 ug.

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  IHC staining of mouse lung using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 15792-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using S100A8 antibody (15792-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of RAW 264.7 using 15792-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human S100A8 (15792-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, A549-Zellen, COLO 320-Zellen, HL-60-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Magenkrebsgewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen
• Erfolgreiche Detektion in FC (Intra): RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 13 publications below
• IF: See 10 publications below

Produktinformation

15792-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA S100A8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A8 fusion protein Ag8500
• Vollständiger Name: S100 calcium binding protein A8
• Berechnetes Molekulargewicht: 93 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC005928
• Gene symbol: S100A8
• Gene ID (NCBI): 6279
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

Publikationen

Species	Application	Title
human	IHC	Cell Metab; QDPR deficiency drives immune suppression in pancreatic cancer; Authors - Ji Liu
human	WB	Mol Cell; Hexokinase 1 cellular localization regulates the metabolic fate of glucose.; Authors - Adam De Jesus
human	WB	Cell Rep Med; Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors; Authors - Yiting Wang
mouse	WB,IHC	Nat Commun; S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.; Authors - Jianjie Li
human	WB	Mol Ther; Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.; Authors - Tian-Jian Yu ; KD Validated
mouse	WB	Phytomedicine; Geniposide via enema alleviates colitis by modulating intestinal flora and bile acid metabolites, inhibiting S100A8/S100A9/NF-κB, and promoting TGR5 inhibition of NLRP3 inflammasome; Authors - Bao-Xin Zheng