S100A8 Rekombinanter Antikörper

S100A8 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

242970C4

Kat-Nr. : 98411-3-RR

Synonyme

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

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  FC experiment of human peripheral blood lymphocytes using 98411-3-RR

  1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4),or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human peripheral blood lymphocytes using 98411-3-RR

  1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98411-3-RR bindet in FC (Intra) S100A8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: S100A8 fusion protein Eg3752
• Vollständiger Name: S100 calcium binding protein A8
• Berechnetes Molekulargewicht: 93 aa, 11 kDa
• GenBank-Zugangsnummer: BC005928
• Gene symbol: S100A8
• Gene ID (NCBI): 6279
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.