S100A8 Rekombinanter Antikörper

S100A8 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

250113D8

Kat-Nr. : CL594-98467-2

Synonyme

Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

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  FC experiment of Transfected HEK-293T using CL594-98467-2

  1x10^6 untransfected or S100A8-transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse S100A8 Rabbit RecAb (CL594-98467-2, Clone: 250113D8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using CL594-98467-2

  1x10^6 untransfected or S100A8-transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse S100A8 Rabbit RecAb (CL594-98467-2, Clone: 250113D8) (red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98467-2 bindet in FC (Intra) S100A8 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: S100A8 fusion protein Eg2959
• Vollständiger Name: S100 calcium binding protein A8 (calgranulin A)
• Berechnetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: NM_013650.2
• Gene symbol: S100a8
• Gene ID (NCBI): 20201
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.