S100A9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A9 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 14226-1-AP

Synonyme

60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14, MRP14, NIF, P14, Protein S100 A9, S100A9

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  WB analysis of mouse spleen using 14226-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14226-1-AP

  WB result of S100A9 antibody (14226-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.

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  WB analysis of human heart using 14226-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 14226-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14226-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 14226-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14226-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14226-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14226-1-AP (S100A9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsillitis using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14226-1-AP (S100A9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14226-1-AP (S100A9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14226-1-AP (S100A9 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, humanes Herzgewebe, MCF-7-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Produktinformation

14226-1-AP bindet in WB, IHC, IF, ELISA S100A9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A9 fusion protein Ag5465
• Vollständiger Name: S100 calcium binding protein A9
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa, 28 kDa
• GenBank-Zugangsnummer: BC047681
• Gene symbol: S100A9
• Gene ID (NCBI): 6280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Publikationen

Species	Application	Title
human	IHC	Cell Metab; QDPR deficiency drives immune suppression in pancreatic cancer; Authors - Ji Liu
human,mouse	IHC,IF	Int J Cancer; S100 calcium-binding protein A9 from tumor-associated macrophage enhances cancer stem cell-like properties of hepatocellular carcinoma.; Authors - Ran Wei
human,mouse	WB,IHC	Cell Biosci; TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling.; Authors - Qiang Liu ; KD Validated
rat	IF	Int J Med Sci; Single-cell RNA Sequencing Uncovered the Involvement of an Endothelial Subset in Neutrophil Recruitment in Chemically Induced Rat Pulmonary Inflammation.; Authors - Hong Huang
human	WB,IF	J Oncol; Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma; Authors - Shiyu Zeng ; KD Validated
human	IHC	Histol Histopathol; Histopathological assessment of calcification and inflammation of calcific aortic valves from patients with and without diabetes mellitus.; Authors - Josephin Mosch