S100B Monoklonaler Antikörper

S100B Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

CloneNo.

1G10F4

Kat-Nr. : 66616-1-Ig

Synonyme

S100 beta, 1G10F4, Protein S100 B, Protein S100-B, S100 calcium-binding protein B



Geprüfte Anwendungen

Erfolgreiche Detektion in WBKaninchenhirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe, Ratten-Rückenmarkgewebe
Erfolgreiche Detektion in IHCRattenhirngewebe, humanes Appendizitis-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PRattenhirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:2000
Immunhistochemie (IHC)IHC : 1:1000-1:8000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66616-1-Ig bindet in WB, IHC, IF-P, ELISA S100B und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen S100B fusion protein Ag7440
Vollständiger Name S100 calcium binding protein B
Berechnetes Molekulargewicht 11 kDa
Beobachtetes Molekulargewicht 11 kDa
GenBank-ZugangsnummerBC001766
Gene symbol S100 Beta
Gene ID (NCBI) 6285
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for S100B antibody 66616-1-IgProtokoll herunterladen
IHC protocol for S100B antibody 66616-1-IgProtokoll herunterladenl
IF protocol for S100B antibody 66616-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB

Acta Neuropathol Commun

Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden

Authors - Vedad Delic
ratIHC

ACS Omega

Platelet-Rich Plasma Gel-Loaded Collagen/Chitosan Composite Film Accelerated Rat Sciatic Nerve Injury Repair

Authors - Bo Yuan
ratIF

Front Neurosci

Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation.

Authors - Bo Jia
humanIF

Transl Oncol

High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.

Authors - Zhigang Wang
ratIF

Exp Neurol

Motor neurons transplantation alleviates neurofibrogenesis during chronic degeneration by reversibly regulating Schwann cells epithelial-mesenchymal transition

Authors - Zhaoyang Wu
WB,IF

Neurochem Res

Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier.

Authors - Liu Xiaoying

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kateryna (Verified Customer) (01-16-2025)

The S100B antibody was utilized to assess the identity of the immunomagnetically isolated astrocytic fraction by Western blot. As a positive control, mouse primary cortical astrocytes were used. As negative controls, mouse primary cortical neurons and microglial cells were used. The samples were processed by SDS-PAGE, and the separated proteins were transferred onto a nitrocellulose membrane. Blocking was performed with 5% non-fat dry milk, and the membrane was incubated overnight at 4°C with the S100B antibody. The antibody is specific and performs consistently.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain astrocytes
S100B Antibody Western Blot validation (1:1000 dilution) in Mouse brain astrocytes (Cat no:66616-1-Ig)
FH

Kateryna (Verified Customer) (11-21-2024)

I use the S100B monoclonal antibody (66616-1-Ig) to validate the identity of immunomagnetically isolated astrocytes from mouse brain via Western blot and as a housekeeping protein for other protein measurements via Western blot. The Western blot is run under reducing conditions using SDS-PAGE, a nitrocellulose membrane, and 5% non-fat dry milk as a blocking solution. The antibody is highly sensitive, providing consistent bands at 11 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain astrocytes
S100B Antibody Western Blot validation (1:1000 dilution) in Mouse brain astrocytes (Cat no:66616-1-Ig)
FH

Reyes (Verified Customer) (07-02-2024)

s100b (in green) works in human brain FFPE tissue, but it does not yield the best results. (It colocalizes in a small way with NeuN in red).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
S100B Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66616-1-Ig)
FH

Gabriele (Verified Customer) (06-06-2024)

The antibody works well, staining positively the hiPSC-derived astrocytes in green (1:200). It can colocalize with GFAP (in red). Nuclei are stained with hoechst (in blue)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: hiPSC-derived Astrocytes
S100B Antibody Immunofluorescence validation (1:200 dilution) in hiPSC-derived Astrocytes (Cat no:66616-1-Ig)
FH

Clarisse (Verified Customer) (08-08-2022)

This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human cerebral organoids
S100B Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:66616-1-Ig)