S100P Polyklonaler Antikörper

S100P Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11803-1-AP

Synonyme

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11803-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11803-1-AP (S100P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 11803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100P antibody (11803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11803-1-AP

  Immunofluorescent analysis of HepG2 cells, using S100P antibody 11803-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 11803-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug S100P antibody (11803-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 5 publications below

Produktinformation

11803-1-AP bindet in WB, IHC, IF, FC, ELISA S100P und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100P fusion protein Ag2379
• Vollständiger Name: S100 calcium binding protein P
• Berechnetes Molekulargewicht: 95 aa, 11 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC006819
• Gene symbol: S100P
• Gene ID (NCBI): 6286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

Publikationen

Species	Application	Title
human	WB, IHC	Am J Cancer Res; High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer.; Authors - Xiangyu Wang
human	IHC	Proteomics Clin Appl; Identification of distinctive protein expression patterns in colorectal adenoma.; Authors - F Lam Francis F
human	IHC	Diagn Pathol; Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion.; Authors - Jung Min Jung MJ
human	WB	Exp Ther Med; Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma.; Authors - Chengyu Wang
human	WB	Technol Cancer Res Treat; LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p; Authors - Zhaozhi Xia
human	IHC	Int J Mol Sci; Expression-Based Diagnosis, Treatment Selection, and Drug Development for Breast Cancer; Authors - Qing Ye