S1PR1/EDG1 Polyklonaler Antikörper

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• KD/KO Validated

S1PR1/EDG1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(49)

Kat-Nr. : 55133-1-AP

Synonyme

S1PR1, EDG1, EDG 1, ECGF1, D1S3362

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  WB analysis using 55133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55133-1-AP (S1PR1/EDG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55133-1-AP

  IP result of anti-S1PR1/EDG1 (IP:55133-1-AP, 4ug; Detection:55133-1-AP 1:300) with mouse brain tissue lysate 2640ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 44 publications below
• IHC: See 10 publications below
• IF: See 12 publications below
• IP: See 4 publications below

Produktinformation

55133-1-AP bindet in WB, IHC, IF, IP, ELISA S1PR1/EDG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sphingosine-1-phosphate receptor 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: NM_001400
• Gene symbol: S1PR1
• Gene ID (NCBI): 1901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S1PR1 (sphingosine-1-phosphate receptor 1), also known as S1P1 or EDG1, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P) (PMID: 18787560). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR1 is widely expressed in various cell types, including endothelial cells and lymphocytes (PMID: 14737169). S1P-S1PR1 plays a major role in lymphocyte egress and chemotaxis, cell proliferation and survival, and tumor angiogenesis and metastasis (PMID: 21102457). The antibody is specific to S1PR1.

Publikationen

Species	Application	Title
mouse	WB,IF	BMC Med; Involvement of sphingosine-1-phosphate receptor 1 in pain insensitivity in a BTBR mouse model of autism spectrum disorder; Authors - Lili Fan
rat	IF	Neural Regen Res; Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury; Authors - Xiao-Yu Zhu
mouse	WB	iScience; Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke; Authors - Yumi Fukuzaki
rat	WB	Int Immunopharmacol; Dexmedetomidine alleviates oxidative stress and mitochondrial dysfunction in diabetic peripheral neuropathy via the microRNA-34a/SIRT2/S1PR1 axis; Authors - Ying Lin
human	IHC, WB, IP	Front Cell Dev Biol; The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.; Authors - Minjeong Yeon
rat	WB	Front Pharmacol; Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.; Authors - Han-Qing Pang