SALL4 Monoklonaler Antikörper

SALL4 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2B5B2

Kat-Nr. : 66236-1-Ig

Synonyme

DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 66236-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66236-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

66236-1-Ig bindet in WB, IHC,ELISA SALL4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SALL4 fusion protein Ag17480
• Vollständiger Name: sal-like 4 (Drosophila)
• Berechnetes Molekulargewicht: 1053 aa, 112 kDa
• Beobachtetes Molekulargewicht: 165 kDa
• GenBank-Zugangsnummer: BC111714
• Gene symbol: SALL4
• Gene ID (NCBI): 57167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta
-catenin and activate the Wnt/beta
-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

Publikationen

Species	Application	Title
human	WB	Cancer Chemother Pharmacol; Sorafenib combined with dasatinib therapy inhibits cell viability, migration, and angiogenesis synergistically in hepatocellular carcinoma.; Authors - Chiung-Chi Cheng
human	IHC	Pediatr Surg Int; SALL4 is a useful marker for pediatric yolk sac tumors.; Authors - Peng Wu