Sam50 Monoklonaler Antikörper

Sam50 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H6H9

Kat-Nr. : 67425-1-Ig

Synonyme

CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

---

Galerie der Validierungsdaten

• 

  WB analysis using 67425-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67425-1-Ig (Sam50 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67425-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67425-1-Ig (Sam50 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67425-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67425-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67425-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67425-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 67425-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Kaninchenherzgewebe, Mausherzgewebe, NIH/3T3-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67425-1-Ig bindet in WB, IHC, IF, ELISA Sam50 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Sam50 fusion protein Ag15155
• Vollständiger Name: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007830
• Gene symbol: SAMM50
• Gene ID (NCBI): 25813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).