SASS6 Polyklonaler Antikörper

SASS6 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21377-1-AP

Synonyme

HsSAS 6, HsSAS-6, SAS 6, SAS6, Spindle assembly abnormal protein 6 homolog

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  WB analysis of HeLa using 21377-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21377-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21377-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21377-1-AP

  IP result of anti-SASS6 (IP:21377-1-AP, 4ug; Detection:21377-1-AP 1:1000) with HeLa cells lysate 600ug.

• 

  IP experiment of HeLa cells using 21377-1-AP

  IP result of anti-SAS6 (21377-1-AP for IP and Detection) with HeLa cells.

• 

  IHC staining of mouse testis using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 21377-1-AP (SASS6 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hodengewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

21377-1-AP bindet in WB, IHC, IF, IP, ELISA SASS6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SASS6 fusion protein Ag15917
• Vollständiger Name: spindle assembly 6 homolog (C. elegans)
• Berechnetes Molekulargewicht: 657 aa, 74 kDa
• Beobachtetes Molekulargewicht: 70-74 kDa
• GenBank-Zugangsnummer: BC101026
• Gene symbol: SASS6
• Gene ID (NCBI): 163786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spindle assembly abnormal protein 6 homolog (SASS6) is a centrosomal protein that, together with PLK4, CEP135, CENPJ (also known as CPAP) and STIL, is involved in regulating the number of centrosomes in human cells.

Publikationen

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
human	WB,IHC	Oncol Rep; Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.; Authors - Lili Du
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	IF	EBioMedicine; Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities; Authors - Niu Li
human	WB	Clin Exp Med; SASS6 promotes tumor proliferation and is associated with TP53 and immune infiltration in lung adenocarcinoma; Authors - Zihao Li