SAT1 Polyklonaler Antikörper

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• KD/KO Validated

SAT1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 10708-1-AP

Synonyme

DC21, Diamine acetyltransferase 1, KFSD, Putrescine acetyltransferase, SAT, SAT1, SSAT, SSAT 1

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 10708-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10708-1-AP (SAT1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10708-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10708-1-AP (SAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10708-1-AP

  IP Result of anti-SAT1 (IP:10708-1-AP, 4ug; Detection:10708-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 10708-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10708-1-AP (SAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

10708-1-AP bindet in WB, IP, IHC, IF, ELISA SAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAT1 fusion protein Ag1154
• Vollständiger Name: spermidine/spermine N1-acetyltransferase 1
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC008424
• Gene symbol: SAT1
• Gene ID (NCBI): 6303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

Publikationen

Species	Application	Title
mouse,human	WB,IHC	Cell Metab; Urea cycle activation triggered by host-microbiota maladaptation driving colorectal tumorigenesis; Authors - Haoyan Chen
human	IHC	Proc Natl Acad Sci U S A; Polyamine pathway contributes to the pathogenesis of Parkinson disease.; Authors - Lewandowski Nicole M NM
rat	WB,IHC	Mol Neurobiol; Electroacupuncture Alleviates Neuropathic Pain by Suppressing Ferroptosis in Dorsal Root Ganglion via SAT1/ALOX15 Signaling; Authors - Kexing Wan
mouse	WB	Food Chem Toxicol; DEHP induces ferroptosis in testes via p38α-lipid ROS circulation and destroys the BTB integrity.; Authors - Ling Yang
mouse	WB	Biochim Biophys Acta Mol Cell Res; Urea transporter B downregulates polyamines levels in melanoma B16 cells via p53 activation.; Authors - Jiajing Li
mouse	WB	PLoS One; Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles.; Authors - Yuanbiao Guo