SBDS Polyklonaler Antikörper

SBDS Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17618-1-AP

Synonyme

CGI 97, SBDS, SDS, SWDS

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Galerie der Validierungsdaten

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  WB analysis using 17618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17618-1-AP (SBDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17618-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17618-1-AP (SBDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17618-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17618-1-AP (SBDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17618-1-AP

  IP Result of anti-SBDS (IP:17618-1-AP, 4ug; Detection:17618-1-AP 1:300) with HL-60 cells lysate 3040ug.

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  IHC staining of human pancreas cancer using 17618-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17618-1-AP (SBDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 17618-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17618-1-AP (SBDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293T-Zellen, HL-60-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17618-1-AP bindet in WB, IP, IHC, IF, ELISA SBDS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SBDS fusion protein Ag11814
• Vollständiger Name: Shwachman-Bodian-Diamond syndrome
• Berechnetes Molekulargewicht: 250 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC065700
• Gene symbol: SBDS
• Gene ID (NCBI): 51119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located. This antibody is a rabbit polyclonal antibody raised against a full-length human SBDS protein, recognizes specifically the 29kd SBDS protein.

Publikationen

Species	Application	Title
human	WB	Oncogenesis; The HIF/PHF8/AR axis promotes prostate cancer progression.; Authors - D Tong
rat	WB	Brain Res Bull; Cerebral ischemia-reperfusion causes a down regulation of HCN1 expression via enhancing the nuclear NRSF-HDAC4 gathering that contributes to neuron damage.; Authors - Pan Luo
human	IHC,IF	Am J Transl Res; Three RNA-binding protein signature as a novel biomarker to predict endometrial cancer prognosis; Authors - Bo Wang