Mammaglobin A Polyklonaler Antikörper

Mammaglobin A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 23501-1-AP

Synonyme

hMAM, Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2

---

• 

  WB analysis of MCF-7 using 23501-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23501-1-AP (Mammaglobin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23501-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23501-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23501-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23501-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

23501-1-AP bindet in WB, IHC, ELISA Mammaglobin A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Mammaglobin A fusion protein Ag20122
• Vollständiger Name: secretoglobin, family 2A, member 2
• Berechnetes Molekulargewicht: 120 aa, 13 kDa
• Beobachtetes Molekulargewicht: 6-10 kDa
• GenBank-Zugangsnummer: BC067220
• Gene symbol: Mammaglobin A
• Gene ID (NCBI): 4250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCGB2A2, also known as mammaglobin A, is a member of the secretoglobin superfamily. It is a breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. SCGB2A2 is a 93-amino acid protein with a calculated molecular weight of 10.5 kDa. This polyclonal antibody is raised against the C-terminal 71 amino acids of the protein encoded by a cDNA (MGC:71974; BC067220).

Publikationen

Species	Application	Title
human	IHC	Mol Med Rep; Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells.; Authors - Bo Liu