SCLT1 Polyklonaler Antikörper

SCLT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 14875-1-AP

Synonyme

Sodium channel-associated protein 1, SAP1, hCAP 1A, CAP1A

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  WB analysis using 14875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14875-1-AP

  IP result of anti-SCLT1 (IP:14875-1-AP, 4ug; Detection:14875-1-AP 1:1000) with human placenta tissue lysate 4000ug.

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  IHC staining of human colon cancer using 14875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14875-1-AP (SCLT1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14875-1-AP (SCLT1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 14875-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green), Centrin 1 antibody (12794-1-AP, orange).

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  IF Staining of hTERT-RPE1 using 14875-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CEP192 antibody (18832-1-AP, orange), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

• 

  IF Staining of hTERT-RPE1 using 14875-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green), CEP250/CNAP1 antibody (14498-1-AP, orange).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:600-1:2400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 7 publications below

Produktinformation

14875-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SCLT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCLT1 fusion protein Ag6646
• Vollständiger Name: sodium channel and clathrin linker 1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 36 kDa,75-80 kDa
• GenBank-Zugangsnummer: BC064428
• Gene symbol: SCLT1
• Gene ID (NCBI): 132320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.; Authors - Hao Yan
human	IF	Nat Commun; Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.; Authors - Qingwen Xu
human	IF	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran