SCP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SCP2 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 23006-1-AP

Synonyme

NLTP, NSL TP, SCP 2, SCP chi, SCP X, SCP2, SCPX, sterol carrier protein 2, Sterol carrier protein X

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Galerie der Validierungsdaten

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  WB analysis using 23006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23006-1-AP

  WB result of SCP2 antibody (23006-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2 transfected HEK-293 cells.

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  WB analysis of K-562 using 23006-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23006-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 23006-1-AP

  IP Result of anti-SCP2 (IP:23006-1-AP, 4ug; Detection:23006-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 23006-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23006-1-AP (SCP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 23006-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SCP2 (23006-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

23006-1-AP bindet in WB, IP, IHC, IF, FC, ELISA SCP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCP2 fusion protein Ag19215
• Vollständiger Name: sterol carrier protein 2
• Berechnetes Molekulargewicht: 547 aa, 59 kDa
• Beobachtetes Molekulargewicht: 13-15 kDa
• GenBank-Zugangsnummer: BC005911
• Gene symbol: SCP2
• Gene ID (NCBI): 6342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.

Publikationen

Species	Application	Title
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
human	WB	iScience; A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells.; Authors - G Jordan Ray
mouse	WB	J Biol Chem; Sterol carrier protein-2 deficiency attenuates diet-induced dyslipidemia and atherosclerosis in mice.; Authors - Honglaing He ; KO Validated
mouse	IF	PLoS One; Sterol Carrier Protein-2, a Nonspecific Lipid-Transfer Protein, in Intracellular Cholesterol Trafficking in Testicular Leydig Cells.; Authors - Nancy C Li
human	WB	J Biochem; Human SCP4 is a chromatin-associated CTD phosphatase and exhibits the dynamic translocation during erythroid differentiation.; Authors - Shotaro Wani
human,rat,rabbit	WB,IHC,IF	Cell Death Discov; SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis; Authors - Tianming Dai