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SCP2/SCPx Polyklonaler Antikörper
SCP2/SCPx Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (2)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23006-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293T-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen |
Erfolgreiche IP | HepG2-Zellen |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:20-1:200 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Produktinformation
23006-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA SCP2/SCPx und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Kaninchen, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | SCP2/SCPx fusion protein Ag19215 |
Vollständiger Name | sterol carrier protein 2 |
Berechnetes Molekulargewicht | 547 aa, 59 kDa |
Beobachtetes Molekulargewicht | 13-15 kDa |
GenBank-Zugangsnummer | BC005911 |
Gene symbol | SCP2 |
Gene ID (NCBI) | 6342 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for SCP2/SCPx antibody 23006-1-AP | Protokoll herunterladen |
IF protocol for SCP2/SCPx antibody 23006-1-AP | Protokoll herunterladen |
IP protocol for SCP2/SCPx antibody 23006-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Phytomedicine Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept | ||
Commun Biol Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism | ||
J Biol Chem Sterol carrier protein-2 deficiency attenuates diet-induced dyslipidemia and atherosclerosis in mice.
| ||
PLoS One Sterol Carrier Protein-2, a Nonspecific Lipid-Transfer Protein, in Intracellular Cholesterol Trafficking in Testicular Leydig Cells. | ||
J Biochem Human SCP4 is a chromatin-associated CTD phosphatase and exhibits the dynamic translocation during erythroid differentiation. |