SDHC Polyklonaler Antikörper

SDHC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14575-1-AP

Synonyme

CYB560, CYBL, PGL3, QPs 1, QPS1, SDH3, SDHC

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Galerie der Validierungsdaten

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  WB analysis using 14575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14575-1-AP (SDHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14575-1-AP (SDHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14575-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14575-1-AP (SDHC antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 14575-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14575-1-AP (SDHC antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Mauslungengewebe, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

14575-1-AP bindet in WB, IHC, ELISA SDHC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDHC fusion protein Ag5775
• Vollständiger Name: succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC033626
• Gene symbol: SDHC
• Gene ID (NCBI): 6391
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDHC(Succinate dehydrogenase cytochrome b560 subunit, mitochondrial) is also named as CYB560, SDH3 and belongs to the cytochrome b560 family. It is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3) and paraganglioma and gastric stromal sarcoma (PGGSS). It has a transit peptide of 29 amino acid and has 5 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	Nucleic Acids Res; Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice.; Authors - Johanna H K Kauppila
mouse	WB	iScience; Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver.; Authors - Tatsuya Yamada
mouse	WB	PLoS One; Quantitative proteomics in A30P*A53T α-synuclein transgenic mice reveals upregulation of Sel1l.; Authors - Jianguo Yan
human	WB	Cell Rep; Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress; Authors - Ying Liu
human	IHC	Front Immunol; Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma; Authors - Han Nan