SEC14L1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SEC14L1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 25541-1-AP

Synonyme

SEC14-like protein 1, SEC14L

---

• 

  WB analysis using 25541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 25541-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 25541-1-AP

  IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:500) with HEK-293 cells lysate 1480 ug.

• 

  IP experiment of HEK-293 using 25541-1-AP

  IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:300) with HEK-293 cells lysate 2600ug.

• 

  IF Staining of A431 using 25541-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SEC14L1 antibody (25541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Y79-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

25541-1-AP bindet in WB, IF/ICC, IP, ELISA SEC14L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC14L1 fusion protein Ag22233
• Vollständiger Name: SEC14-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 719 aa, 82 kDa
• Beobachtetes Molekulargewicht: 81 kDa, 77 kDa
• GenBank-Zugangsnummer: BC142979
• Gene symbol: SEC14L1
• Gene ID (NCBI): 6397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC14L1 (SEC14-like protein 1) is a member of SEC14 family in mammals with the potential to control local phospholipid content in membranes. SEC14L1 may act as a phospholipid transfer protein. It has also been reported that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling and is involved in innate immunity (PMID: 17092608; 23843640). SEC14L1 has three isoforms with the molecular mass of 81.2kDa, 81.8kDa and 77.1kDa.

Publikationen

Species	Application	Title
human	WB	Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta ; KO Validated
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi