SEC24D Monoklonaler Antikörper

SEC24D Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G5G9

Kat-Nr. : 67409-1-Ig

Synonyme

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

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  WB analysis of Jurkat using 67409-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67409-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67409-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67409-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67409-1-Ig bindet in WB, IF/ICC, ELISA SEC24D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SEC24D fusion protein Ag4657
• Vollständiger Name: SEC24 family, member D (S. cerevisiae)
• Berechnetes Molekulargewicht: 1033 aa, 113 kDa
• Beobachtetes Molekulargewicht: 113 kDa
• GenBank-Zugangsnummer: BC035761
• Gene symbol: SEC24D
• Gene ID (NCBI): 9871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).