SEL1L Polyklonaler Antikörper

SEL1L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29801-1-AP

Synonyme

Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L, Suppressor of lin-12-like protein 1

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  WB analysis using 29801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29801-1-AP (SEL1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29801-1-AP (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 29801-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 29801-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, BxPC-3-Zellen, HEK-293T-Zellen, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29801-1-AP bindet in WB, IHC, ELISA SEL1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEL1L fusion protein Ag32178
• Vollständiger Name: sel-1 suppressor of lin-12-like (C. elegans)
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: NM_005065
• Gene symbol: SEL1L
• Gene ID (NCBI): 6400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen.