SEMA4A Polyklonaler Antikörper

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• KD/KO Validated

SEMA4A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 27359-1-AP

Synonyme

CORD10, RP35, Sema B, SEMA4A, SEMAB, Semaphorin 4A, Semaphorin B, SEMB

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 27359-1-AP

  WB result of SEMA4A antibody (27359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEMA4A transfected HeLa cells.

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  WB analysis using 27359-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27359-1-AP (SEMA4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27359-1-AP bindet in WB, IHC, ELISA SEMA4A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEMA4A fusion protein Ag26023
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• Berechnetes Molekulargewicht: 761 aa, 84 kDa
• Beobachtetes Molekulargewicht: 120-150 kDa
• GenBank-Zugangsnummer: BC020974
• Gene symbol: SEMA4A
• Gene ID (NCBI): 64218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sema4A, the membrane-bound class 4 semaphorin, belongs to semaphorins superfamily. Sema4A funtions as potent axonal repellants in the nervous system, and is involved in vascular development. Two species of Sema4A with molecular weights of about 150 and 120 kDa were observed. The antibody recognizes the Sema4A protein, which is of appropriate molecular size (~120 kDa) for the glycosylated protein(PMID: 26296689/17318185/26024919).

Publikationen

Species	Application	Title
human	WB	J Cancer; SEMA4A promotes prostate cancer invasion: involvement of tumor microenvironment; Authors - Xiao Liu