SEMA4F Polyklonaler Antikörper

SEMA4F Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12474-1-AP

Synonyme

M SEMA, m Sema M, PRO2353, Sema M, Sema W, SEMA4F, SEMAM, Semaphorin 4F, Semaphorin M, Semaphorin W, SEMAW

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 12474-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12474-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12474-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12474-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12474-1-AP

  IP Result of anti-SEMA4F (IP:12474-1-AP, 4ug; Detection:12474-1-AP 1:500) with HepG2 cells lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HepG2-Zellen, humanes Lebergewebe, Maushirngewebe, Y79-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12474-1-AP bindet in WB, IP,ELISA SEMA4F und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEMA4F fusion protein Ag3082
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
• Berechnetes Molekulargewicht: 615 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 84 kDa
• GenBank-Zugangsnummer: BC018361
• Gene symbol: SEMA4F
• Gene ID (NCBI): 10505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.