SENP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SENP1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 25349-1-AP

Synonyme

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1

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  WB analysis using 25349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25349-1-AP (SENP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 25349-1-AP

  IP result of anti-SENP1 (IP:25349-1-AP, 4ug; Detection:25349-1-AP 1:1000) with Jurkat cells lysate 1400 ug.

• 

  IHC staining of human ovary cancer using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 25349-1-AP (SENP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

25349-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA SENP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SENP1 fusion protein Ag18028
• Vollständiger Name: SUMO1/sentrin specific peptidase 1
• Berechnetes Molekulargewicht: 644 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73-74 kDa
• GenBank-Zugangsnummer: BC045639
• Gene symbol: SENP1
• Gene ID (NCBI): 29843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Publikationen

Species	Application	Title
human	WB	Nature; Lactate regulates cell cycle by remodeling the anaphase promoting complex; Authors - Weihai Liu ; KD Validated
human,mouse	WB	Free Radic Biol Med; Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion; Authors - Kang Xia
human	WB	Int Immunopharmacol; Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma; Authors - Jiayuan Liang
human	WB, IHC	Cancer Med; Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1.; Authors - Mingyue Zhou
human	WB	J Pharmacol Sci; Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.; Authors - Fang Xianjun ; KD Validated
	WB	J Virol; Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication; Authors - Xiangju Wu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This is a great antibody for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa