SERPINA10 Polyklonaler Antikörper

SERPINA10 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11851-1-AP

Synonyme

PZI, Serpin A10, SERPINA10, ZPI

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Galerie der Validierungsdaten

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  WB analysis of human liver using 11851-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11851-1-AP

  IP Result of anti-SERPINA10 (IP:11851-1-AP, 3ug; Detection:11851-1-AP 1:1000) with L02 cells lysate 2500ug.

• 

  IHC staining of human liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 11851-1-AP

  Immunofluorescent analysis of L02 cells using 11851-1-AP (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11851-1-AP

  Immunofluorescent analysis of HepG2 cells, using SERPINA10 antibody 11851-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HepG2-Zellen, L02-Zellen, Mauslebergewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: L02-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11851-1-AP bindet in WB, IP, IHC, IF, ELISA SERPINA10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERPINA10 fusion protein Ag2420
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• Berechnetes Molekulargewicht: 444 aa, 51 kDa
• Beobachtetes Molekulargewicht: 72 kDa, 68 kDa, 50-55 kDa
• GenBank-Zugangsnummer: BC022261
• Gene symbol: SERPINA10
• Gene ID (NCBI): 51156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.