Maspin Polyklonaler Antikörper

Maspin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11722-1-AP

Synonyme

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 11722-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11722-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11722-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11722-1-AP (Maspin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 11722-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Maspin antibody (11722-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 11722-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human Maspin (11722-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11722-1-AP bindet in WB, IHC, IF, FC, ELISA Maspin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Maspin fusion protein Ag2304
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC020713
• Gene symbol: SERPINB5
• Gene ID (NCBI): 5268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

Publikationen

Species	Application	Title
human	IHC	Int J Cancer; Serpin B5 is a CEA-interacting biomarker for colorectal cancer.; Authors - Baek Ji Yeon JY
human	WB	Cancer Lett; Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute to radioresistance in nasopharyngeal carcinoma by suppressing the p53-mediated apoptosis pathway.; Authors - Chang-Fu Yang
human	WB	Oncol Lett; iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers.; Authors - Hengwei Zhang
human	IF	Cell Death Dis; FAT10 promotes chemotherapeutic resistance in pancreatic cancer by inducing epithelial-mesenchymal transition via stabilization of FOXM1 expression.; Authors - Jinfeng Zhu