SERPINB6 Polyklonaler Antikörper

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• KD/KO Validated

SERPINB6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 14962-1-AP

Synonyme

PI6, PTI, Serpin B6, PI-6, PI 6

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  WB analysis using 14962-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14962-1-AP (SERPINB6 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14962-1-AP

  WB result of SERPINB6 antibody (14962-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SERPINB6 transfected HeLa cells.

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  WB analysis of mouse skeletal muscle using 14962-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14962-1-AP (SERPINB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14962-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14962-1-AP (SERPINB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14962-1-AP

  IP result of anti-SERPINB6 (IP:14962-1-AP, 4ug; Detection:14962-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human skeletal muscle using 14962-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14962-1-AP (SERPINB6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 14962-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14962-1-AP (SERPINB6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14962-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SERPINB6 antibody (14962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:18000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

14962-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SERPINB6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERPINB6 fusion protein Ag6824
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 6
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC001394
• Gene symbol: SERPINB6
• Gene ID (NCBI): 5269
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Syst Biol; Proteome-wide copy-number estimation from transcriptomics; Authors - Andrew J Sweatt
mouse	WB	Cell Tissue Res; Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouse submandibular gland.; Authors - Hiroki Nakata
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
mouse	WB	PLoS One; New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease.; Authors - Flora Cozzolino
human	WB,IHC	Cell Mol Gastroenterol Hepatol; Single-cell transcriptomics reveals early molecular and immune alterations underlying the serrated neoplasia pathway toward colorectal cancer; Authors - Yu-Jie Zhou