SERPINE2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SERPINE2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 11303-1-AP

Synonyme

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

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Galerie der Validierungsdaten

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  WB analysis of C6 using 11303-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11303-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11303-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11303-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11303-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11303-1-AP

  IP Result of anti-SERPINE2 (IP:11303-1-AP, 3ug; Detection:11303-1-AP 1:300) with A549 cells lysate 2800ug.

• 

  IHC staining of human testis using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas cancer using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11303-1-AP (SERPINE2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SERPINE2 antibody (11303-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, A549-Zellen, MCF-7-Zellen, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Hodengewebe, A549-Zellen, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Produktinformation

11303-1-AP bindet in WB, IP, IHC, IF, ELISA SERPINE2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERPINE2 fusion protein Ag1830
• Vollständiger Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC015663
• Gene symbol: SERPINE2
• Gene ID (NCBI): 5270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Publikationen

Species	Application	Title
human	WB,IHC,IP	Cancer Lett; SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.; Authors - Jingjing Zhang ; KD Validated
human	WB,IHC	Respir Res; High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma.; Authors - Ryota Dokuni ; KD Validated
human	WB	J Immunol; Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes.; Authors - Shengquan Tang
human	IP	Front Cell Dev Biol; BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.; Authors - Xiyang Zhang
mouse	IF	Sci Rep; PMID: 30446696; Authors - Mary Ann Stepp
human	IHC	J Cell Physiol; Transcriptional profiling of endometriosis tissues identifies genes related to organogenesis defects.; Authors - Crispi Stefania S

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ahmad (Verified Customer) (06-18-2021)

Fix in 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, 0.02% NP-40 for 1 hr works good for staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: rabbit cornea

SERPINE2 Antibody Immunofluorescence validation (1:300 dilution) in rabbit cornea (Cat no:11303-1-AP)