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  • KD/KO Validated

SERPINE2 Polyklonaler Antikörper

SERPINE2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 11303-1-AP

Synonyme

PI7, PN1, Serpin E2, GDN, Glia derived nexin



Geprüfte Anwendungen

Erfolgreiche Detektion in WBC6-Zellen, A549-Zellen, MCF-7-Zellen, Rattenhirngewebe, Rattenherzgewebe
Erfolgreiche IPA549-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe, humanes Pankreaskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Hodengewebe, Maushodengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11303-1-AP bindet in WB, IHC, IF-P, IP, ELISA SERPINE2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SERPINE2 fusion protein Ag1830
Vollständiger Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht 44-50 kDa
GenBank-ZugangsnummerBC015663
Gene symbol SERPINE2
Gene ID (NCBI) 5270
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SERPINE2 antibody 11303-1-APProtokoll herunterladen
IHC protocol for SERPINE2 antibody 11303-1-APProtokoll herunterladenl
IF protocol for SERPINE2 antibody 11303-1-APProtokoll herunterladen
IP protocol for SERPINE2 antibody 11303-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
humanWB,IP,IHC

Cancer Lett

SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

Authors - Jingjing Zhang
  • KD Validated
humanWB,IHC

Respir Res

High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma.

Authors - Ryota Dokuni
  • KD Validated

J Cereb Blood Flow Metab

Agomelatine promotes differentiation of oligodendrocyte precursor cells and preserves white matter integrity after cerebral ischemic stroke

Authors - Shisi Wang
humanWB

J Immunol

Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes.

Authors - Shengquan Tang
humanIP

Front Cell Dev Biol

BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.

Authors - Xiyang Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ahmad (Verified Customer) (06-18-2021)

Fix in 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, 0.02% NP-40 for 1 hr works good for staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: rabbit cornea
SERPINE2 Antibody Immunofluorescence validation (1:300 dilution) in rabbit cornea (Cat no:11303-1-AP)