HSP47/SERPINH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HSP47/SERPINH1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (3)

Kat-Nr. : 10875-1-AP

Synonyme

HSP47, SERPINH1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3

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  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10875-1-AP

  WB result of HSP47 antibody (10875-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47 transfected HeLa cells.

• 

  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47/SERPINH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10875-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10875-1-AP (HSP47 antibody at dilution of 1:50.

• 

  IF Staining of HeLa using 10875-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (10875-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10875-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP47 (10875-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, A2780-Zellen, A431-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolongewebe, humanes Leberzirrhosegewebe, humanes Lungenkarzinomgewebe, Mausnierengewebe, Mauslungengewebe, Rattennierengewebe, Rattenlungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

10875-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA HSP47/SERPINH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSP47/SERPINH1 fusion protein Ag1301
• Vollständiger Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC014623
• Gene symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47, which is required for the correct folding and secretion of collagen (PMID: 30128672). SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease, keloid scars, and lung fibrosis (PMID: 32091407). SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines (PMID: 24595468). SERPINH1 regulates the Wnt/β-catenin signaling pathway and modulates gastric cancer (GC) progression (PMID: 32091407). SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer (PMID: 32091407). SERPINH1 promotes invasion and metastasis of breast cancer cells by regulating the expression of several extracellular matrix (ECM) proteins (PMID: 25744716). Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Protoc; Responsive and activable nanomedicines for remodeling the tumor microenvironment.; Authors - Yinlong Zhang ; KD Validated
human	WB,IF,IHC	Nat Commun; Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.; Authors - Xuexiang Han ; KD Validated
human,mouse	WB,IHC	Cancer Res; RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.; Authors - Nicholas Rooney
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gohar (Verified Customer) (02-06-2020)

na

• Applications: Immunohistochemistry,

FH

Gohar (Verified Customer) (02-06-2020)

na

• Applications: Immunohistochemistry,

FH

Danielle (Verified Customer) (09-09-2019)

This antibody, as well as the other 5 purchased for our purposes, was sufficient and provided clear results for our experiment.

• Applications: ChIP,