SETD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SETD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 55377-1-AP

Synonyme

EC:2.1.1.-, EC:2.1.1.359, HIF 1, HIF1, HIF-1

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  WB analysis using 55377-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55377-1-AP (SETD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS cells using 55377-1-AP

  WB result of SETD2 antibody (55377-1-AP, 1:500) with siRNA transfected U2-OS cells.

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  WB analysis of Jurkat using 55377-1-AP

  WB result of SETD2 antibody (55377-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and SETD2 knockout Jurkat cells.

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  IP experiment of Jurkat using 55377-1-AP

  IP result of anti-SETD2 (IP:55377-1-AP, 4ug; Detection:55377-1-AP 1:800) with Jurkat cells lysate 1360 ug.

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  IHC staining of human colon cancer using 55377-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 55377-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55377-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD2 antibody (55377-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55377-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SETD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SET domain containing 2
• Berechnetes Molekulargewicht: 288 kDa
• Beobachtetes Molekulargewicht: 287 kDa
• GenBank-Zugangsnummer: NM_014159
• Gene symbol: SETD2
• Gene ID (NCBI): 29072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.; Authors - Ruiqiong Liu ; KD Validated
mouse,human	WB,IHC	Cell Death Dis; Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC; Authors - Wei Xue ; KD Validated
human		Carcinogenesis; Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer.; Authors - Jie Gao
human	WB	J Exp Clin Cancer Res; A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression.; Authors - Wentao Liu
human	WB,CoIP	Function (Oxf); Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination; Authors - Gaurav Kumar