SF3A2 Polyklonaler Antikörper

SF3A2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 15596-1-AP

Synonyme

PRP11, PRPF11, SAP 62, SAP62, SF3A2, SF3a66, Splicing factor 3A subunit 2

---

• 

  WB analysis of NIH/3T3 using 15596-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15596-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15596-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 15596-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15596-1-AP (SF3A2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 15596-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15596-1-AP (SF3A2 antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15596-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA SF3A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SF3A2 fusion protein Ag7953
• Vollständiger Name: splicing factor 3a, subunit 2, 66kDa
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC004434
• Gene symbol: SF3A2
• Gene ID (NCBI): 8175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
rat	WB,IF,CoIP	J Adv Res; Ginsenoside Rb2 inhibits p300-mediated SF3A2 acetylation at lysine 10 to promote Fscn1 alternative splicing against myocardial ischemic/reperfusion injury; Authors - Qingxia Huang
mouse	WB	iScience; Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice; Authors - Han Wang