SF3A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SF3A3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12070-1-AP

Synonyme

PRP9, PRPF9, SAP 61, SAP61, SF3A3, SF3a60, Splicing factor 3A subunit 3

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Galerie der Validierungsdaten

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  WB analysis using 12070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12070-1-AP

  WB result of SF3A3 antibody (12070-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SF3A3 transfected A549 cells.

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  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 12070-1-AP

  IP Result of anti-SF3A3 (IP:12070-1-AP, 4ug; Detection:12070-1-AP 1:600) with Raji cells lysate 2000ug.

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  IHC staining of human lung cancer using 12070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12070-1-AP (SF3A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12070-1-AP

  Immunofluorescent analysis of Hela cells, using SF3A3 antibody 12070-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, K-562-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12070-1-AP bindet in WB, IP, IHC, IF, ELISA SF3A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SF3A3 fusion protein Ag2708
• Vollständiger Name: splicing factor 3a, subunit 3, 60kDa
• Berechnetes Molekulargewicht: 501 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC011523
• Gene symbol: SF3A3
• Gene ID (NCBI): 10946
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
bovine	WB	Int J Mol Sci; Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress; Authors - Jianguo Liu