SFI1 Polyklonaler Antikörper

SFI1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13550-1-AP

Synonyme

hSFI1, hSfi1p, KIAA0542, PISD, Protein SFI1 homolog, RP5 858B16.1, SFI1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 13550-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13550-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13550-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13550-1-AP

  Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13550-1-AP

  Immunofluorescent analysis of Hela cells, using SFI1 antibody 13550-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

13550-1-AP bindet in WB, IF, ELISA SFI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFI1 fusion protein Ag4268
• Vollständiger Name: Sfi1 homolog, spindle assembly associated (yeast)
• Berechnetes Molekulargewicht: 1211 aa, 134 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: BC033613
• Gene symbol: SFI1
• Gene ID (NCBI): 9814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL.; Authors - Andrew Kodani
human	WB	Sci Signal; Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins.; Authors - Isha Nasa
mouse	WB	Sci Rep; Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation.; Authors - Akiko Matsuura
human	WB	J Cell Sci; Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.; Authors - Ebtissal M Khouj
human	IF	EMBO J; Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis; Authors - Marine H Laporte ; KD Validated