Surfactant Protein A Polyklonaler Antikörper

Surfactant Protein A Polyklonal Antikörper für FC, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11850-1-AP

Synonyme

Alveolar proteinosis protein, COLEC4, Collectin 4, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SFTPA2, SP A, SP A1, Surfactant Protein A, surfactant protein A1

---

Galerie der Validierungsdaten

• 

  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 11850-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11850-1-AP (Surfactant Protein A antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of A549 using 11850-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Surfactant Protein A (11850-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lungenkarzinomgewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

11850-1-AP bindet in WB, IHC, FC, ELISA Surfactant Protein A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Surfactant Protein A fusion protein Ag2417
• Vollständiger Name: surfactant protein A1
• Berechnetes Molekulargewicht: 248 aa, 26 kDa
• GenBank-Zugangsnummer: BC026229
• Gene symbol: SFTPA1
• Gene ID (NCBI): 653509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

Publikationen

Species	Application	Title
pig	WB	Sci Total Environ; Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets; Authors - Xiaotong Wang
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human,mouse	IHC	Cancer Immunol Immunother; SFTPA1 is a potential prognostic biomarker correlated with immune cell infiltration and response to immunotherapy in lung adenocarcinoma.; Authors - Lu Yuan
mouse	WB	Biochem Biophys Res Commun; Increased mortality from influenza infection in long-chain acyl-CoA dehydrogenase knockout mice.; Authors - Apurva Shinde
human	WB	Environ Toxicol; Silica nanoparticle exposure inhibits surfactant protein A and B in A549 cells through ROS-mediated JNK/c-Jun signaling pathway.; Authors - Xiaojing Yang
mouse	WB	J Thorac Dis; Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice; Authors - Yueming Wang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C