SFXN1 Monoklonaler Antikörper

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• KD/KO Validated

SFXN1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D9F1

Kat-Nr. : 68024-1-Ig

Synonyme

2D9F1, sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein

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  WB analysis using 68024-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of MCF-7 using 68024-1-Ig

  WB result of SFXN1 antibody (68024-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN1 transfected MCF-7 cells.

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  IF Staining of MCF-7 using 68024-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, MOLT-4-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68024-1-Ig bindet in WB, IF/ICC, ELISA SFXN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SFXN1 fusion protein Ag2985
• Vollständiger Name: sideroflexin 1
• Berechnetes Molekulargewicht: 35.6 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC020517
• Gene symbol: SFXN1
• Gene ID (NCBI): 94081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.