Beta Sarcoglycan Polyklonaler Antikörper

Beta Sarcoglycan Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 11936-1-AP

Synonyme

43DAG, A3b, Beta sarcoglycan, Beta SG, LGMD2E, SGC, SGCB

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 11936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 11936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

11936-1-AP bindet in WB, IHC, IF, ELISA Beta Sarcoglycan und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Sarcoglycan fusion protein Ag2533
• Vollständiger Name: sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
• Berechnetes Molekulargewicht: 318 aa, 35 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC020709
• Gene symbol: SGCB
• Gene ID (NCBI): 6443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SGCB is a 43-kD component of the dystrophin-glycoprotein complex.

Publikationen

Species	Application	Title
rat	WB	Phytomedicine; Cardioprotective effect of icariin against myocardial fibrosis and its molecular mechanism in diabetic cardiomyopathy based on network pharmacology: Role of ICA in DCM.; Authors - Liping Zhang
mouse	WB,IF	Oncotarget; Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.; Authors - Endong Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Venkata (Verified Customer) (01-03-2022)

I have loaded 50 and 100ug of protein in each of the lanes and the samples are from mouse heart and skeletal muscle tissues. I don't see expected bands in all the samples and most of the bands are non specific.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse cardiac and skeletal muscle

Beta Sarcoglycan Antibody Western Blot validation (1:500 dilution) in Mouse cardiac and skeletal muscle (Cat no:11936-1-AP)