SGCG Polyklonaler Antikörper

SGCG Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18102-1-AP

Synonyme

35DAG, A4, DAGA4, DMDA, DMDA1, Gamma sarcoglycan, Gamma SG, LGMD2C, MAM, SCARMD2, SCG3, SGCG, TYPE

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 18102-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 18102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18102-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18102-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 18102-1-AP

  IP Result of anti-SGCG (IP:18102-1-AP, 4ug; Detection:18102-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human heart using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

18102-1-AP bindet in WB, IP, IHC, IF, ELISA SGCG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGCG fusion protein Ag12402
• Vollständiger Name: sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
• Berechnetes Molekulargewicht: 291 aa, 32 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC074777
• Gene symbol: SGCG
• Gene ID (NCBI): 6445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF,IP	Skelet Muscle; Novel γ-sarcoglycan interactors in murine muscle membranes.; Authors - Tara C Smith
mouse	IHC	PLoS One; Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.; Authors - Alison M Blain
human	IF	PLoS One; Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies.; Authors - Narinder Janghra
human	IF	Hum Gene Ther Methods; Optimisation of internally deleted dystrophin constructs.; Authors - Mojgan Reza
mouse	IHC	Mol Vis; Identification of HMX1 target genes: a predictive promoter model approach.; Authors - Boulling Arnaud A