SGSH Polyklonaler Antikörper

SGSH Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14184-1-AP

Synonyme

HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14184-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14184-1-AP (SGSH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14184-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14184-1-AP (SGSH Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IHC staining of human pancreas cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Transfected
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14184-1-AP bindet in WB, IHC,ELISA SGSH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGSH fusion protein Ag5435
• Vollständiger Name: N-sulfoglucosamine sulfohydrolase
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC047318
• Gene symbol: SGSH
• Gene ID (NCBI): 6448
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SGSH(N-sulphoglucosamine sulphohydrolase) is also named as HSS, sulphamidase and belongs to the sulfatase family. Sulfamidase is synthesized as a 62 kDa precursor protein, which is modified with mannose 6-phosphate (M6P) residues, allowing their recognition by mannose-6-phosphate receptors in the Golgi complex and ensuring transport to the endosomal/lysosomal system(PMID:21671382). It catalyzes the third step of degradation of glucosaminoglycans and is required for the degradation of heparan sulphate. Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A), also known as Sanfilippo syndrome A.