Bif-1 Polyklonaler Antikörper

Bif-1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15422-1-AP

Synonyme

Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 15422-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 15422-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15422-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15422-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15422-1-AP

  Immunofluorescent analysis of HepG2 cells, using SH3GLB1 antibody 15422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, humanes Plazenta-Gewebe, Mausherzgewebe, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15422-1-AP bindet in WB, IHC, IF, ChIP, ELISA Bif-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Bif-1 fusion protein Ag7710
• Vollständiger Name: SH3-domain GRB2-like endophilin B1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC007455
• Gene symbol: SH3GLB1
• Gene ID (NCBI): 51100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IHC	Eur J Pharmacol; SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.; Authors - Anbo Gao
human	WB	J Exp Clin Cancer Res; SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.; Authors - Chia-Hung Chien
mouse	WB	Oncotarget; Autophagy-related gene expression is an independent prognostic indicator of glioma.; Authors - Huixue Zhang
human	WB,ChIP	BMC Cancer; Role of SH3GLB1 in the regulation of CD133 expression in GBM cells; Authors - Chia-Hung Chien