SH3GLB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SH3GLB2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15897-1-AP

Synonyme

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 15897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15897-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15897-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 15897-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15897-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15897-1-AP

  IP Result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.

• 

  IF Staining of HeLa using 15897-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hodengewebe, Maushodengewebe, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

15897-1-AP bindet in WB, IP, IF, ELISA SH3GLB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH3GLB2 fusion protein Ag8712
• Vollständiger Name: SH3-domain GRB2-like endophilin B2
• Berechnetes Molekulargewicht: 395 aa, 44 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC014635
• Gene symbol: SH3GLB2
• Gene ID (NCBI): 56904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

Publikationen

Species	Application	Title
human	WB	Brain Pathol; Neuronal Susceptibility to Beta-Amyloid Toxicity and Ischemic Injury Involves Histone Deacetylase-2 Regulation of Endophilin-B1.; Authors - David B Wang
mouse	WB,IF	J Biol Chem; Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.; Authors - Jacob M Serfass ; KO Validated