SH3PXD2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SH3PXD2A Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 18976-1-AP

Synonyme

Adapter protein TKS5, FISH, KIAA0418, SH3 and PX domains 2A, SH3 multiple domains protein 1, SH3MD1, SH3PXD2A, TKS5

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18976-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18976-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 18976-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 18976-1-AP

  Immunofluorescent analysis of HepG2 cells using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

18976-1-AP bindet in WB, IHC, IF, CoIP, ELISA SH3PXD2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SH3 and PX domains 2A
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: NM_014631
• Gene symbol: SH3PXD2A
• Gene ID (NCBI): 9644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts (PMID: 19464300). The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150 kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells (PMID: 9687503). This antibody is specific to SH3PXD2A.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.; Authors - Shu-Chen Liu
mouse	IF	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	IF	Adv Healthc Mater; Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration; Authors - Zhao Wei
		Theranostics; IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.; Authors - Ge Liu
human	WB	Oncotarget; The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.; Authors - Shinji Iizuka ; KD Validated
human	WB,IHC,IF	J Oncol; Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma; Authors - Koroku Kato ; KD Validated

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