SIL1 Polyklonaler Antikörper

SIL1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24110-1-AP

Synonyme

UNQ545/PRO836, ULG5, MSS, BiP-associated protein, BiP associated protein

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  WB analysis using 24110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24110-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse colon using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24110-1-AP

  Immunofluorescent analysis of HepG2 cells using 24110-1-AP (SIL1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Mausnierengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maus-Kolongewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

24110-1-AP bindet in WB, IHC, IF/ICC, ELISA SIL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIL1 fusion protein Ag21367
• Vollständiger Name: SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC011568
• Gene symbol: SIL1
• Gene ID (NCBI): 64374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.; Authors - Francesca Potenza
mouse	WB	Mol Biol Cell; Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion.; Authors - Viraj P Ichhaporia
mouse	WB	Mol Neurobiol; Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration; Authors - Yuanyuan Xu