SIP1 Polyklonaler Antikörper

SIP1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21672-1-AP

Synonyme

Component of gems 2, Gemin 2, GEMIN2, SIP1, SIP1 delta, SMN interacting protein 1

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  WB analysis of HepG2 using 21672-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21672-1-AP (SIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21672-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21672-1-AP (SIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21672-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21672-1-AP (SIP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21672-1-AP

  IP result of anti-SIP1 (IP:21672-1-AP, 4ug; Detection:21672-1-AP 1:500) with HepG2 cells lysate 1000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

21672-1-AP bindet in WB, IP, ELISA SIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIP1 fusion protein Ag16500
• Vollständiger Name: survival of motor neuron protein interacting protein 1
• Berechnetes Molekulargewicht: 280 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC104968
• Gene symbol: SIP1
• Gene ID (NCBI): 8487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.; Authors - Alessia Mirra
human		Neurobiol Dis; UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.; Authors - Simona Rossi
human	WB	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang
human	WB	Biomolecules; (20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2.; Authors - Yu-Shi Wang